Characterization of the cytotoxic effect of a chimeric restriction enzyme, H1o-FokI

Resistance of Cloned 1F5 Chimeric Anti-CD20 Antibody Heavy-Chain Gene to DNA Polymerase due to a Predicted Hairpin Structure

Background: Formation of secondary structure such as DNA hairpins or loops may influence molecular genetics methods and PCR based approaches necessary for genetic engineering, in addition to gene regulation. Materials and Methods: A polymerase chain reaction with splice overlap extension (SOE-PCR) was used to create fully synthetic 1F5 chimeric anti-CD20 heavy- and light-chain genes. The chi...

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA

A Bi-Functional Targeted P28-NRC Chimeric Protein with Enhanced Cytotoxic Effects on Breast Cancer Cell Lines

One of the emerging therapeutic strategies for targeted therapy of cancer is the use of chimeric proteins. The p28 peptide has the ability of selective entrance and activating apoptosis in breast cancer cells. The NRC antimicrobial peptide showed cytotoxic activity on various breast cancer including drug-resistant variants but also normal cell lines. Here we designed a chimeric protein consiste...

A Bi-Functional Targeted P28-NRC Chimeric Protein with Enhanced Cytotoxic Effects on Breast Cancer Cell Lines

One of the emerging therapeutic strategies for targeted therapy of cancer is the use of chimeric proteins. The p28 peptide has the ability of selective entrance and activating apoptosis in breast cancer cells. The NRC antimicrobial peptide showed cytotoxic activity on various breast cancer including drug-resistant variants but also normal cell lines. Here we designed a chimeric protein consiste...

FokI and BsmI Polymorphisms of the VDR Gene and Breast Cancer Risk

Introduction: Vitamin D fulfills its crucial role in cell proliferation and death through signal transduction into the nucleus by vitamin D receptor (VDR). Recent studies have depicted the association between VDR gene polymorphisms and different cancers, including breast cancer. This study attempted to consider the relationship between VDR gene polymorphisms and breast cancer risk among women i...